Journal: Cell Discovery

Article Title: Regulation of the divalent metal ion transporter via membrane budding

doi: 10.1038/celldisc.2016.11

Figure Lengend Snippet: Endogenous DMT1 is released in EVs from mouse gut explants. ( A ) Endogenous DMT1 is released in EVs by mouse gut explants under normal and high iron conditions. CD26 is used as a loading control. ( B ) Densitometry quantification of DMT1 release in EVs from the gut normalized against CD26 shows a trend for increase in the amount of DMT1 released under high iron conditions. Data are mean±s.e.m.; n =4 animals per group. ( C ) Quantitative PCR (Q-PCR) shows a ~8.5-fold increase in the expression of Arrdc4 in the duodenum of mice fed a high iron diet compared with normal iron diet. The TATA box binding protein was used as the reference gene. Data are mean±s.e.m., n =3–4, ** P <0.01. ( D ) DMT1 is released in gut EVs from WT and Arrdc1 −/− mice. ( E ) Densitometry quantification of DMT1 release in EVs from the gut normalized against CD26 shows that the levels of DMT1 released in gut EVs is not changed in Arrdc1 −/− compared with WT mice. ( F ) The protein concentration of EVs released from Arrdc1 −/− gut EVs is the same as from WT gut EVs. Data are mean±s.e.m.; n =4 animals per group. ( G ) DMT1 is released in gut EVs from WT and Arrdc4 −/− mice. ( H ) The levels of DMT1 released in gut EVs is not changed in Arrdc4 −/− compared with WT mice. ( I ) The protein concentration of EVs released from Arrdc4 −/− gut EVs is significantly reduced compared with WT gut EVs. Data are mean±s.e.m.; n =4 animals per group. ** P <0.01.

Article Snippet: Sources of commercial antibodies were as follows: rat monoclonal anti-HA (clone 3F10) and mouse monoclonal anti-c-myc (clone 9E10) from Roche Diagnostics (Indianapolis, IN, USA); rabbit polyclonal anti-GFP (clone ab290), mouse monoclonal anti-CD63 (MEM 259), goat polyclonal Myc (ab9132), rabbit polyclonal anti-CD26 (ab28340) and rabbit anti-LAMP1 from Abcam (Cambridge, MA, USA); mouse anti-flotillin-2 antibody (clone 29), mouse anti-γ adaptin, mouse anti-GM130 from BD Biosciences (San Jose, CA, USA); mouse monoclonal anti-β-actin (clone AC-15), rat monoclonal anti-Flag (clone 6F7), mouse monoclonal anti-Flag (clone M2) and rabbit polyclonal anti-Tsg101 (Sigma-Aldrich, St Louis, MO, USA), goat polyclonal anti-GFP from Rockland Immunochemicals (Limerick, PA, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Binding Assay, Protein Concentration

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Emodin was found to inhibit DPP4 activity after screening a natural compound library.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Activity Assay, Drug discovery

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Anthraquinone compounds inhibit DPP4 activity.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Activity Assay, Inhibition, Binding Assay

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Docking model reveals the binding mode of emodin to DPP4 protein.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Binding Assay

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Emodin treatment (3, 10 and 30 mg/kg, P.O.) in mice decreased the plasma DPP4 activity in a dose-dependent manner.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Clinical Proteomics, Activity Assay

Journal: Frontiers in bioscience (Landmark edition)

Article Title: DPP4 Regulates the Th17/IL-17 Axis and Accelerates Epithelial Mesenchymal Transition to Promote Ovalbumin-Induced Asthma in Female C57BL/6J Mice.

doi: 10.31083/j.fbl2812342

Figure Lengend Snippet: Fig. 1. sCD26/sDPP4 promoted Th17 polarization and the secretion of IL-17. CD4+ T cells were induced differentiation into Th17 cells and treated with sCD26/sDPP4 or dipeptidyl peptidase-4 (DPP4) inhibitor. (A,B) The ratio of Th17 cells was detected by flow

Article Snippet: The levels of IL-17 (CSB-E04608m) in the cell supernatant and DPP4 (CSB-E08520m), IL-17 (CSBE04608m), IL-4 (CSB-E04634m), IL-5 (CSB-E04637m), IL-13 (CSB-E04602m), TGF-β1 (CSB-E04726m), MMP9 (CSB-E08007m) in BALF were detected by ELISA kit (CUSABIO, Wuhan, China).

Techniques:

Journal: Frontiers in bioscience (Landmark edition)

Article Title: DPP4 Regulates the Th17/IL-17 Axis and Accelerates Epithelial Mesenchymal Transition to Promote Ovalbumin-Induced Asthma in Female C57BL/6J Mice.

doi: 10.31083/j.fbl2812342

Figure Lengend Snippet: Fig. 4. Overexpression of DPP4 promoted airway inflammation in asthmatic mice. (A) The level of DPP4 in the bronchoalveolar lavage fluid (BALF) was analyzed by ELISA. (B) The total number of leukocytes in the BALF was calculated by a hemocytometer.

Article Snippet: The levels of IL-17 (CSB-E04608m) in the cell supernatant and DPP4 (CSB-E08520m), IL-17 (CSBE04608m), IL-4 (CSB-E04634m), IL-5 (CSB-E04637m), IL-13 (CSB-E04602m), TGF-β1 (CSB-E04726m), MMP9 (CSB-E08007m) in BALF were detected by ELISA kit (CUSABIO, Wuhan, China).

Techniques: Over Expression, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in bioscience (Landmark edition)

Article Title: DPP4 Regulates the Th17/IL-17 Axis and Accelerates Epithelial Mesenchymal Transition to Promote Ovalbumin-Induced Asthma in Female C57BL/6J Mice.

doi: 10.31083/j.fbl2812342

Figure Lengend Snippet: Fig. 5. Overexpression of DPP4 promoted airway epithelial mesenchymal transition (EMT) in OVA-induced asthmatic mice. (A)

Article Snippet: The levels of IL-17 (CSB-E04608m) in the cell supernatant and DPP4 (CSB-E08520m), IL-17 (CSBE04608m), IL-4 (CSB-E04634m), IL-5 (CSB-E04637m), IL-13 (CSB-E04602m), TGF-β1 (CSB-E04726m), MMP9 (CSB-E08007m) in BALF were detected by ELISA kit (CUSABIO, Wuhan, China).

Techniques: Over Expression